Accession PRJCA001353
Title The Chinese Sacbrood Virus
Relevance Agricultural
Data Types Metagenome
Organisms Bacteria
Description Honeybees are important pollinators and play an important role in ecosystem stability. Honeybee populations have fallen sharply in recent years. Viruses are one of the potential causes of colony collapse disorder (CCD) in honeybees. Chinese Sacbrood virus (CSBV) leads to mass death of honeybee larvae. However, there is little research on the molecular response of honeybees to Chinese Sacbrood Virus (CSBV) disease. Here, we used RNA-seq to sequence infected and uninfected Chinese honeybee larvae. Our results showed that the immune response of infected larvae was strongly increased, four antimicrobial peptides were significantly up-regulated, two major genes Dicer-like and AGO2 in the RNAi antiviral response pathway were also significantly highly expressed, and some genes in the signal pathway related to bee immunity were significantly differentially expressed in the infected honeybee larvae. Interestingly, we found a significant decrease in T4 bacteriophage in infected larvae. These results suggest that CSBV activated immune-related signaling pathways in Chinese honeybee larvae, with responses to CSBV infection involving regulation of certain key genes in these pathways. Our investigation provides important insights into a molecular basis for further research on host-virus interaction mechanisms.
Sample scope Environment
Release date 2019-03-29
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 31572338
Submitter Jilian    Li  (bumblebeeljl@hotmail.com)
Organization Institute of Apicultural Research, Chinese Academy of Agricultual Sciences
Submission date 2019-03-29

Project Data

Resource name Description
BioSample (19) -
SAMC068463 RNA-seq of honeybee larvae: Apis cerana
SAMC068462 RNA-seq of honeybee larvae: Apis cerana
SAMC068461 RNA-seq of honeybee larvae: Apis cerana
SAMC068460 RNA-seq of honeybee larvae: Apis cerana
SAMC068459 RNA-seq of honeybee larvae: Apis cerana
SAMC068458 RNA-seq of honeybee larvae: Apis cerana
SAMC068457 RNA-seq of honeybee larvae: Apis cerana
SAMC068456 RNA-seq of honeybee larvae: Apis cerana
SAMC068455 RNA-seq of honeybee larvae: Apis cerana
SAMC068454 RNA-seq of honeybee larvae: Apis cerana
SAMC068453 RNA-seq of honeybee larvae: Apis cerana
SAMC068452 RNA-seq of honeybee larvae: Apis cerana
SAMC068451 RNA-seq of honeybee larvae: Apis cerana
SAMC068450 RNA-seq of honeybee larvae: Apis cerana
SAMC068449 RNA-seq of honeybee larvae: Apis cerana
SAMC068448 RNA-seq of honeybee larvae: Apis cerana
SAMC068447 RNA-seq of honeybee larvae: Apis cerana
SAMC068446 RNA-seq of honeybee larvae: Apis cerana
SAMC068445 RNA-seq of honeybee larvae: Apis cerana
GSA (1) -
CRA001558 The transcriptome of honeybee larvae (Apis cerana) reveals mechanisms of antiviral immunity
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