Accession PRJCA001329
Title Full-length transcriptome sequencing of the longissimus dorsi of buffalo and cattle
Relevance Agricultural
Data Types Transcriptome or Gene expression
Raw sequence reads
Organisms Bos taurus
Bubalus bubalis
Description Six longissimus muscle samples from Guangxi native cattles and swamp buffalo ( adult, 12 M old) were collected from nanning meat factory abattoir, a local slaughterhouse of Nanning, P.R. China. Transcriptome sequencing have been identified in various tissues and cell types from human, monkey, porcine and mouse. However, expression profile of Total RNA across Guangxi native cattle and swamp buffalo muscle development has never been investigated. Here, we examine the expression of full-length transcriptome sequencing in cattle and buffalo muscle at adult stage(12 months), exhibiting the first report of lncRNA in the Guangxi native cattle and swamp buffalo muscle development of a large animal. 16,236 lncRNA candidates were obtained from buffalo skeletal muscle samples, of which a number of lncRNAs were highly abundant, and 2,161 lncRNAs were differentially expressed between buffalo and cattle. Real-time quantitative PCR (qPCR) analysis confirmed the expression profile of these lncRNAs, including several highly abundant lncRNAs, and a subset of differently expressed lncRNAs according to the high-throughput RNA sequencing (RNA-seq) data. These results indicate that abundant lncRNA is differentially expressed in bovine muscle, indicating important and diverse functions in mammalian muscle development.
Sample scope Multispecies
Release date 2019-04-03
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 31860638
Submitter Huang    kongwei   (893968979@qq.com)
Organization Guangxi University
Submission date 2019-03-14

Project Data

Resource name Description
BioSample (6) -
SAMC064003 Buffalo-3
SAMC064002 Buffalo-2
SAMC064001 Buffalo-1
SAMC063999 Cattle-03
SAMC063998 Cattle-2
SAMC063997 Cattle-1
GSA (1) -
CRA001488 Bovine transcriptome
-