Description |
Kluyveromyces marxianus, the known fastest-growing eukaryote on Earth, has remarkable thermotolerance and capacity to utilize various agricultural residues to produce low cost bioethanol, hence is industrially important to resolve the imminent energy shortage crisis. Currently, the poor ethanol tolerance hinders its operable application on industry, and it is necessary to improve K. marxianus' ethanol resistance and unravel the underlying systematical mechanisms. However, this has been seldomly reported to date. We carried out the wild-type haploid K. marxianus in an adaptive ethanol evolution. After one hundred-day evolution, the evolved population was obtained, and its ethanol tolerance increased. To identify the DNA variation between pre- and post-evolution, we performed DNA-seq analysis, and present the data here. |