Accession PRJCA001267
Title An Arabidopsis secondary metabolite targets bacterial virulence for defense
Relevance Model organism
Data types Metagenome
Organisms Microbiota
Pseudomonas syringae
Description Plants deploy a variety of seconday metabolites to fend off pathogen attack. While it is generally believed that defense compounds are toxic or inhibitory to microbes, the exact mechanisms are often unknown. Here we show that Arabidopsis plants produce compounds that selectively inhibit Pseudomonas syringae virulence gene expression, rather than inhibit bacterial growth or viability. Through bioassay-guided purification, structural elucidation, and chemical synthesis, we demonstrate that one such compound is sulforaphane, a derivative of aliphatic glucosinolate. At physiological concentrations, SFN preferentially inhibits the expression of P. syringae genes encoding the type III secretion system in vitro and in planta. Plants defective in SFN production is unable to attenuate bacterial type III gene expression and exhibit increased susceptibility to P. syringae bacteria. The selectivity of SFN to P. syringae virulence is further supported by the findings that in Arabidopsis plants SFN does not impact a P. syringae mutant lacking the type III system and leaf-associated bacterial microbiota. We further performed target profiling and mutagenesis analyses to show that SFN attenuates P. syringae virulence by specifically targeting Cys209 of HrpS, a key transcription factor controlling type III gene expression, and a mutation in Cys209 renders the bacterium insensitive to SFN.
Sample scope Multispecies
Release date 2019-02-02
Agency program Grant ID Grant title
No funding support
Submitter Yong-Xin    Liu  (
Organization Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
Submission date 2019-02-02

Project Data

Resource name Description
BioSample (57)  show -
GSA (3) -
CRA002319 Pseudomonas RNA-Seq data
CRA001772 Pseudomonas RNA-Seq data
CRA001771 Arabidopsis leaf microbiota 16S amplicon data