Accession PRJCA001267
Title An Arabidopsis secondary metabolite targets bacterial virulence for defense
Relevance Model organism
Data Types Metagenome
Organisms Microbiota
Pseudomonas syringae
Description Plants deploy a variety of seconday metabolites to fend off pathogen attack. While it is generally believed that defense compounds are toxic or inhibitory to microbes, the exact mechanisms are often unknown. Here we show that Arabidopsis plants produce compounds that selectively inhibit Pseudomonas syringae virulence gene expression, rather than inhibit bacterial growth or viability. Through bioassay-guided purification, structural elucidation, and chemical synthesis, we demonstrate that one such compound is sulforaphane, a derivative of aliphatic glucosinolate. At physiological concentrations, SFN preferentially inhibits the expression of P. syringae genes encoding the type III secretion system in vitro and in planta. Plants defective in SFN production is unable to attenuate bacterial type III gene expression and exhibit increased susceptibility to P. syringae bacteria. The selectivity of SFN to P. syringae virulence is further supported by the findings that in Arabidopsis plants SFN does not impact a P. syringae mutant lacking the type III system and leaf-associated bacterial microbiota. We further performed target profiling and mutagenesis analyses to show that SFN attenuates P. syringae virulence by specifically targeting Cys209 of HrpS, a key transcription factor controlling type III gene expression, and a mutation in Cys209 renders the bacterium insensitive to SFN.
Sample scope Multispecies
Release date 2019-02-02
Grants
Agency program Grant ID Grant title
No funding support
Submitter Yong-Xin    Liu  (woodcorpse@163.com)
Organization Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
Submission date 2019-02-02

Project Data

Resource name Description
BioSample (51) -
SAMC072601 SFN treatment SNF_3
SAMC072600 SFN treatment SNF_2
SAMC072599 SFN treatment SNF_1
SAMC072598 DMSO treatment mock_3
SAMC072597 DMSO treatment mock_2
SAMC072596 DMSO treatment mock_1
SAMC072595 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R12
SAMC072594 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R11
SAMC072593 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R10
SAMC072592 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R09
SAMC072591 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R08
SAMC072590 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R07
SAMC072589 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R06
SAMC072588 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R05
SAMC072587 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R04
SAMC072586 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R03
SAMC072585 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R02
SAMC072584 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R01
SAMC072583 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R11
SAMC072582 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R10
SAMC072581 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R08
SAMC072580 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R07
SAMC072579 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R06
SAMC072578 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R05
SAMC072577 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R04
SAMC072576 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R03
SAMC072575 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R02
SAMC072574 16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R01
SAMC072573 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R12
SAMC072572 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R11
SAMC072571 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R09
SAMC072570 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R08
SAMC072569 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R07
SAMC072568 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R06
SAMC072567 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R05
SAMC072566 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R04
SAMC072565 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R03
SAMC072564 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R02
SAMC072563 16S amplicon sequencing of plant leaf microbiome sampleMybTot1R01
SAMC072562 16S amplicon sequencing of plant leaf microbiome sampleColTot1R12
SAMC072561 16S amplicon sequencing of plant leaf microbiome sampleColTot1R11
SAMC072560 16S amplicon sequencing of plant leaf microbiome sampleColTot1R10
SAMC072559 16S amplicon sequencing of plant leaf microbiome sampleColTot1R09
SAMC072558 16S amplicon sequencing of plant leaf microbiome sampleColTot1R08
SAMC072557 16S amplicon sequencing of plant leaf microbiome sampleColTot1R07
SAMC072556 16S amplicon sequencing of plant leaf microbiome sampleColTot1R06
SAMC072555 16S amplicon sequencing of plant leaf microbiome sampleColTot1R05
SAMC072554 16S amplicon sequencing of plant leaf microbiome sampleColTot1R04
SAMC072553 16S amplicon sequencing of plant leaf microbiome sampleColTot1R03
SAMC072552 16S amplicon sequencing of plant leaf microbiome sample ColTot1R02
SAMC072551 16S amplicon sequencing of plant leaf microbiome sample ColTot1R01
GSA (2) -
CRA001772 Pseudomonas RNA-Seq data
CRA001771 Arabidopsis leaf microbiota 16S amplicon data
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