Accession PRJCA001245
Title Epigenomic Profiling Identifies the Role of Nr5a2 and CYP1B1 in Hepatocellular Carcinoma Stemness Maintenance and Drug Resistance
Relevance Medical
Data Types Epigenomics
Transcriptome or Gene expression
Raw sequence reads
Organisms Homo sapiens
Description In this project, we utilized Side Population (SP) assay to enrich CSCs from human HCC Huh7 cell line. Compared with the non-SP (NSP) cells, the Huh7 SP cells showed stronger abilities of proliferation, colony-formation and invasion, suggesting that the Huh7 SP cells possessed CSCs properties. RNA-seq analysis and RT-qPCR confirmed the Huh7 SP cells maintained a distinctive stemness gene expression signature. Based on the integrated analysis of Hi-C, ATAC-seq together with public ChIP-seq sources, we found that the SP cells preferentially harbored motifs for transcription factor (TF) Nr5a2 and Klf13, and we also identified 12 promising candidate genes, which were up-regulated in the SP cells. Furthermore, our data suggested a potential role of Nr5a2 in the stemness maintenance and drug resistance of CSCs by increasing the expression of CYP1B1. Together, our work highlights the utility of epigenomic profiling to uncover the mechanism of gene regulation in CSCs and provides promising drug targets for HCC targeted gene therapy.
Sample scope Monoisolate
Release date 2019-10-01
Publication
PubMed ID Article title Journal name DOI Year
Epigenomic profiling identifies the role of Nr5a2 and CYP1B1 in hepatocellular carcinoma stemness maintenance Science Bulletin 10.1016/j.scib.2019.06.017 2019
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) Young Scientists Fund 81701765
Anhui Provincial Natural Science Foundation 1808085MH295
Submitter Xiaoyuan    Song  (songxy5@ustc.edu.cn)
Organization University of Science and Technology of China
Submission date 2019-01-25

Project Data

Resource name Description
BioSample (12) -
SAMC065127 Huh7_NSP2_RNA-seq
SAMC065126 Huh7_NSP1_RNA-seq
SAMC065125 Huh7_SP2_RNA-seq
SAMC065124 Huh7_SP1_RNA-seq
SAMC065123 Huh7_NSP2_ATAC-seq
SAMC065122 Huh7_NSP1_ATAC-seq
SAMC065121 Huh7_SP2_ATAC-seq
SAMC065120 Huh7_SP1_ATAC-seq
SAMC065119 Huh7_NSP2_HiC
SAMC065118 Huh7_NSP1_HiC
SAMC065117 Huh7_SP2_HiC
SAMC065116 Huh7_SP1_HiC
GSA (1) -
CRA001409 Epigenomic Profiling Identifies the Role of Nr5a2 and CYP1B1 in Hepatocellular Carcinoma Stemness Maintenance and Drug Resistance
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