Accession PRJCA001125
Title Derivation of mouse haploid trophoblast stem cells
Data Types Transcriptome or Gene expression
Organisms Mus musculus
Description Trophoblast stem (TS) cells are increasingly used as a model system for studying placentation and placental disorders. However, practical limitations of genetic manipulation have posed challenges for genetic analysis using TS cells. Here, we report the generation of mouse parthenogenetic haploid TS cells (haTSCs), and show that supplementation with FGF4 and inhibition of Rho-associated protein kinase (ROCK) enables the maintenance of their haploidy and developmental potential. The resulting haTSCs have 20 chromosomes, exhibit typical expression features of TS cells, possess the multipotency to differentiate into specialized trophoblast cell types, and can chimerize E13.5 and term placentas. We also demonstrate the capability of the haTSCs to undergo genetic manipulation and facilitate genome-wide screening in the trophoblast lineage. We expect that haTSCs will offer a powerful tool for studying functional genomics and placental biology.
Sample scope Monoisolate
Release date 2019-01-09
Grants
Agency program Grant ID Grant title
Chinese Academy of Sciences (CAS) Strategic Priority Research Program of Chinese Academy of Sciences (CAS) XDA16030400
Submitter Guihai    Feng  (fenggh@aliyun.com)
Organization Institute of Zoology, Chinese Academy of Sciences
Submission date 2018-11-17

Project Data

Resource name Description
BioSample (6) -
SAMC048031 RNA_Seq
SAMC048030 RNA_Seq
SAMC048029 RNA_Seq
SAMC048028 RNA_Seq
SAMC048027 RNA_Seq
SAMC048026 RNA_Seq
GSA (1) -
CRA001220 haTSC_data
-