Accession PRJCA001116
Title mRNA 5-methylcytosine mediates pulmonary fibrosis in mouse after PM2.5 exposure
Relevance Environmental
Data Types Epigenomics
Transcriptome or Gene expression
Raw sequence reads
Organisms Mus musculus
Description Airborne particulate matter (PM) with an aerodynamic diameter less than 2.5 um (PM2.5) associates with multiple airway diseases, which mainly ingests through the respiratory tract and is able to enter the lungs, extremely likely inducing pulmonary fibrosis. However, the precise molecular mechanisms of PM2.5-induced pulmonary fibrosis is the subject of intense investigation and currently poorly understood. Herein, we found that PM2.5 exposure leads to aberrant mRNA 5-methylcytosine (m5C) gain and loss in pulmonary fibrosis mouse lung. Further, we first show the m5C mediated regulatory map of gene ontology and protein protein interaction after PM2.5 exposure. atp5j2, atp5l, atpif1, ndufb6, chi3l1, lcn2, mmp9, adipoq, fgr, slc11a1 and tyrobp, which are highly enriched in oxidative stress response, inflammatory response and immune system process and act as m5C gain-upregulated factors, are critical for development of PM2.5-induced fibrosis in mouse lung. Our study manifests a novel mechanism of RNA m5C methylation-regulated PM2.5-induced pulmonary fibrosis development and paves the way for identification and application of potential biomarkers of lung injury after PM2.5 exposure in further study.
Sample scope Monoisolate
Release date 2019-10-24
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) U1432245
Submitter sun    baofa  (sunbf@big.ac.cn)
Organization Beijing Institute of Genomics, Chinese Academy of Sciences
Submission date 2018-11-13

Project Data

Resource name Description
BioSample (4) -
SAMC048002 PM-12W_RNA-Seq
SAMC048001 C-12W_RNA-Seq
SAMC048000 PM-12W-BS_RNA-BisSeq
SAMC047999 C-12W-BS_RNA-BisSeq
GSA (1) -
CRA001230 mRNA 5-methylcytosine mediates pulmonary fibrosis in mouse after PM2.5 exposure