| Accession | PRJCA001013 | ||||||||||||||||
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| Title | RNA m5C prevents maternal mRNA decay during zebrafish embryogenesis | ||||||||||||||||
| Relevance | Model organism | ||||||||||||||||
| Data types |
Epigenomics
Transcriptome or Gene expression |
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| Organisms | Danio rerio | ||||||||||||||||
| Description | The maternal-to-zygotic transition (MZT) is a conserved and fundamental progression during which the embryos undergoes dramatic reprogramming to convert the maternal environment to embryonic-driven programing. However, how the maternally supplied transcripts are dynamically regulated during MZT remains largely unknown. Herein, through genome-wide profiling of RNA 5-methylcytosine (m5C) in zebrafish early embryos, we show that m5C methylated maternal mRNAs display higher stability during MZT. We identify the Y box-binding protein 1 (Ybx1) as a novel m5C reader specifically binding to m5C-modified mRNAs through the vital amino acid Trp45 in its cold shock domain (CSD). Ybx1 is essential for maternal mRNA stability and early embryogenesis in zebrafish. Cooperated with an mRNA stabilizer Pabpc1a, Ybx1 promotes the stability of its target mRNAs in an m5C-dependent manner. Our study demonstrates a novel mechanism of RNA m5C methylation-regulated maternal mRNA stability during zebrafish MZT, highlighting the critical role of m5C mRNA methylation in early development. | ||||||||||||||||
| Sample scope | Multispecies | ||||||||||||||||
| Release date | 2023-03-10 | ||||||||||||||||
| Grants |
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| Submitter | sun baofa (sunbf@big.ac.cn) | ||||||||||||||||
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences | ||||||||||||||||
| Submission date | 2018-08-30 |