Accession PRJCA000979
Title Single-cell RNA-seq of HeLa cells
Relevance Evolution
Data types Transcriptome or Gene expression
Raw sequence reads
Organisms Homo sapiens
Description DNA replication causes the dosage imbalance in every cell cycle. Although acetylated histones (H3K56ac) can incorporate into newly replicated DNA regions and suppress their expression, this compensatory mechanism cannot completely restore the dosage balance. In this study, we used single-cell transcriptome analyses to estimate the extent of dosage compensation between early- and late-replicating genes in mammalian cells. We found a global increase in expression level of early-replicating genes during the mid-S phase, resulting in a dosage imbalance between early- and late-replicating genes in HeLa cells. We proposed a synchronized replication hypothesis that genes encoding subunits of the same protein complex are replicated simultaneously so that the dosage balance within a protein complex is warranted. Our study reveals that the demand for dosage balance during S phase plays an important role in the optimization of the replication-timing program.
Sample scope Single cell
Release date 2019-09-22
PubMed ID Article title Journal name DOI Year
31662304 Synchronized replication of genes encoding the same protein complex in fast-proliferating cells Genome Research 10.1101/gr.254342.119 2019
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 91731302
Submitter ying    chen  (
Organization Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
Submission date 2018-08-09

Project Data

Resource name Description
BioSample (1) -
SAMC044700 HeLa single cell
GSA (1) -
CRA001055 Single-cell RNA-seq of HeLa cells