| Description |
The structural variation (SV) exert important functional impacts on biological phenotypic diversities. Taking prodeoxyviolacein (PDV) biosynthesis as an example, we showed a ring synthetic yeast chromosome V (ring_synV) can be used to continuously generate complex genomic variations and improve the production of PDV by applying SCRaMbLE in haploid yeast cells. The SCRaMbLE of ring_synV is able to generate aneuploid yeast strains with increased PDV productivity, and we identified aneuploid chromosome I, III, VI, XII, XIII, as well as ring_synV. The neochromosome of SCRaMbLEd ring_synV generated more unbalanced form of variations, including duplication, insertions and balanced forms of translocations and inversions than its linear form. Furthermore, of the 27 novel SV detected, 11 prompted the PDV biosynthesis; and the deletion of uncharacterized gene YER182W is related with the promotion of the PDV. Overall, we showed the scrambling ring_synV embraces the evolution of genome by modifying the chromosome number, structure and organization, and evolution of organisms with diverse properties, identifying targets for phenotypic comprehension. |
