Accession PRJCA000897
Title Genomic rearrangement
Data Types Whole genome sequencing
Raw sequence reads
Organisms Saccharomyces cerevisiae
Description The structural variation (SV) exert important functional impacts on biological phenotypic diversities. Taking prodeoxyviolacein (PDV) biosynthesis as an example, we showed a ring synthetic yeast chromosome V (ring_synV) can be used to continuously generate complex genomic variations and improve the production of PDV by applying SCRaMbLE in haploid yeast cells. The SCRaMbLE of ring_synV is able to generate aneuploid yeast strains with increased PDV productivity, and we identified aneuploid chromosome I, III, VI, XII, XIII, as well as ring_synV. The neochromosome of SCRaMbLEd ring_synV generated more unbalanced form of variations, including duplication, insertions and balanced forms of translocations and inversions than its linear form. Furthermore, of the 27 novel SV detected, 11 prompted the PDV biosynthesis; and the deletion of uncharacterized gene YER182W is related with the promotion of the PDV. Overall, we showed the scrambling ring_synV embraces the evolution of genome by modifying the chromosome number, structure and organization, and evolution of organisms with diverse properties, identifying targets for phenotypic comprehension.
Sample scope Monoisolate
Release date 2018-09-06
Submitter Zexiong    Xie  (xzx_tju@163.com)
Organization Tianjin University
Submission date 2018-06-02

Project Data

Resource name Description
BioSample (6) -
SAMC042967 yWJS321
SAMC042966 yWJS184
SAMC042965 yWJS067
SAMC042964 yWJS047
SAMC042963 yWJS044
SAMC042962 yWJS1
GSA (1) -
CRA000963 ring_synV
-