| Accession | PRJCA000889 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Title | monkey off-target | ||||||||
| Relevance | genetics | ||||||||
| Umbrella project |
eGPS: evolutionary Genotype-Phenotype Systems biology Cranial Capacity & Structure Regulation of primate brain structure using Talen/Cas9 modified key genes |
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| Data types | Whole genome sequencing | ||||||||
| Organisms | Macaca mulatta | ||||||||
| Description | The CRISPR-Cas9 system is a highly efficient genome editing tool widely used in model and non-model animals with promising potential in human gene therapy. Although with much improvement, the off-target effect of CRISPR-Cas9 remains a concern. Non-human primates (NHPs) share close genetic, immunological, physiological and neural similarity with humans, making them the ideal preclinical models for developing Cas9-based therapies. However, no robust off-target assessment has been conducted in NHPs. Here we performed trio sequencing of five Cas9-treated monkeys and their wild type parents to detect off-target effect of CRISPR-Cas9 system. | ||||||||
| Sample scope | Multiisolate | ||||||||
| Release date | 2018-10-01 | ||||||||
| Grants |
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| Submitter | Xin Luo (luoxin@mail.kiz.ac.cn) | ||||||||
| Organization | Kunming Institute of Zoology, Chinese Academy of Sciences | ||||||||
| Submission date | 2018-05-24 |