Accession PRJCA000878
Title Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming
Data type Whole genome sequencing
Epigenomics
Raw sequence reads
Genome sequencing
Organism Mus musculus
Description We profile 36,199 single-cell transcriptomes at multiple timepoints throughout a highly efficient chemical reprogramming system using RNA-sequencing and reconstruct their progression trajectories. Through identifying sequential molecular events, we reveal that the dynamic early embryonic-like programs account for successful reprogramming from XEN-like state to pluripotency, including the transcriptomic signature of concomitant two-cell (2C) embryonic-like and early pluripotency program and the epigenetic signature of notable genome-wide DNA demethylation. Moreover, via enhancing the 2C-like program by fine-tuning chemical treatment, the reprogramming process is remarkably accelerated. Collectively, our findings offer a high-resolution dissection of cell fate dynamics during chemical reprogramming and shed light on new mechanistic insights into the nature of induced pluripotency.
Sample scope Single cell
Release date 2018-06-25
Publication
PubMed ID Article title Journal name DOI Year
Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming Cell Stem Cell 10.1016/j.stem.2018.05.025 2018
Submitter Yifang    Liu  (liuyifang3000@gmail.com)
Organization Peking University
Submission date 2018-05-14

Project Data

Resource name Description
BioSample (30) -
SAMC036637 WGBS_XEN-like cells
SAMC036636 WGBS_CiPSCs
SAMC036635 WGBS_pZscan4c-Em-
SAMC036634 WGBS_pZscan4c-Em+
SAMC036633 RNA-Seq_OG+PDGFRAlow/-PECAM-
SAMC036632 RNA-Seq_Control
SAMC036631 RNA-Seq_Opt. VPA
SAMC036630 RNA-Seq_FBS/KSR-SII condition
SAMC036629 RNA-Seq_CiPSCs#1
SAMC036628 RNA-Seq_ESCs
SAMC036627 RNA-Seq_SIII D21
SAMC036626 RNA-Seq_SIII D15
SAMC036625 RNA-Seq_SIII D6 (Unsorted)
SAMC036624 RNA-Seq_SII D12 (N2B27-SII condition)
SAMC036623 RNA-Seq_XEN-like cells
SAMC036622 RNA-Seq_MEFs
SAMC036621 RNA-Seq_pZscan4c-Em-
SAMC036620 RNA-Seq_pZscan4c-Em+
SAMC036619 scRNA-Seq_ESCs
SAMC036618 scRNA-Seq_SIII D21
SAMC036617 scRNA-Seq_SIII D15
SAMC036616 scRNA-Seq_SIII D10
SAMC036615 scRNA-Seq_SIII D8
SAMC036614 scRNA-Seq_SIII D6
SAMC036613 scRNA-Seq_SIII D3
SAMC036612 scRNA-Seq_SII D12
SAMC036611 scRNA-Seq_SII D8
SAMC036610 scRNA-Seq_SI D12
SAMC036609 scRNA-Seq_SI D5
SAMC036608 scRNA-Seq_MEF, XEN-like cells and CiPSCs
GSA (1) -
CRA000932 Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming
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