| Accession | PRJCA000878 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Title | Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming | ||||||||||
| Relevance | Medical | ||||||||||
| Data types |
Whole genome sequencing
Epigenomics Raw sequence reads Genome sequencing |
||||||||||
| Organisms | Mus musculus | ||||||||||
| Description | We profile 36,199 single-cell transcriptomes at multiple timepoints throughout a highly efficient chemical reprogramming system using RNA-sequencing and reconstruct their progression trajectories. Through identifying sequential molecular events, we reveal that the dynamic early embryonic-like programs account for successful reprogramming from XEN-like state to pluripotency, including the transcriptomic signature of concomitant two-cell (2C) embryonic-like and early pluripotency program and the epigenetic signature of notable genome-wide DNA demethylation. Moreover, via enhancing the 2C-like program by fine-tuning chemical treatment, the reprogramming process is remarkably accelerated. Collectively, our findings offer a high-resolution dissection of cell fate dynamics during chemical reprogramming and shed light on new mechanistic insights into the nature of induced pluripotency. | ||||||||||
| Sample scope | Single cell | ||||||||||
| Release date | 2018-06-25 | ||||||||||
| Publication |
|
||||||||||
| Submitter | Yifang Liu (liuyifang3000@gmail.com) | ||||||||||
| Organization | Peking University | ||||||||||
| Submission date | 2018-05-14 |