| Accession | PRJCA000877 |
|---|---|
| Title | Exploring mechanisms of chemaical-induced mouse hepatocarcinogenesis using time serial transcriptomes, small RNA expression profiles and dynamic regulatory networks |
| Relevance | Medical |
| Data types |
Transcriptome or Gene expression
Raw sequence reads |
| Organisms | Mus musculus |
| Description | Hepatocellular carcinoma is one of the malignant tumor which threatening human health. The exact pathogenesis is still not clear and it lack of effective therapeutic targets. To identify the expression profiles of microRNA (miRNA), transcription factor (TF), protein-coding gene and investigate the regulatory network, firstly we constructed diethylnitrosamine (DEN) - induced mouse model and sequenced the normal tissues, inflammatory tissues, tumor tissues and para-tumor tissues of mouse liver through RNA-sequencing and small RNA-sequencing. Differentially expressed genes (DEGs) including Cyp2c29 and regulation of prominent pathways were identified. Functional validation of Cyp2c29 were performed through overexpression trial in mice liver of Acetaminophen (APAP) and carbon tetrachloride (CCl4) models. In addition, the gene expression profiles of APAP liver injury model were also identified using RNA-seq. The further goal is using dynamic miRNA/TF regulatory networks to explore the underlying mechanisms of HCC. |
| Sample scope | tissue |
| Release date | 2019-02-26 |
| Submitter | Qin Tang (tangqinbioinfo@gmail.com) |
| Organization | Huazhong University of Science and Technology |
| Submission date | 2018-05-12 |