Accession PRJCA000770
Title Improving prodeoxyviolacein production via Multiplex SCRaMbLE Iterative Cycles
Relevance Evolution
Data Types Whole genome sequencing
Organisms Saccharomyces cerevisiae
Description Synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system have been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with desired phenotype, Multiplex SCRaMbLE Iterative Cycles (MuSIC) strategy is developed for screening of high PDV production strains. Whole genome sequencing analysis reveals large duplications, deletions, and even entire chromosomes synV duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates artificial DNA rearrangement can be used to accelerate microbial evolution and production of bio-based chemicals.
Sample scope Synthetic
Release date 2018-06-01
Submitter Juan    Wang  (919669315@qq.com)
Organization Tianjin University
Submission date 2018-02-24

Project Data

Resource name Description
BioSample (6) -
SAMC021105 yWJR104
SAMC021104 yWJR085
SAMC021103 yWJR040
SAMC021102 yWJR026
SAMC021101 yWJR006
SAMC021100 yWJR001
GSA (1) -
CRA000772 Improving prodeoxyviolacein production via Multiplex SCRaMbLE Iterative Cycles
-