Accession PRJCA000532
Title HONO denitrification
Relevance Environmental
Data types Transcriptome or Gene expression
Genome sequencing
Organisms Proteobacteria
Description The composition of the Earth's atmosphere is strongly affected by the biogeochemical cycling of reactive nitrogen species. Nitrous acid, HONO, is a key species due to its effect on free radical formation and recycling. At low soil water content, HONO is released by aerobic ammonia-oxidizing bacteria, but little is known about HONO release in water-saturated and oxygen-limited soils. We find large HONO emissions from soils at high water content (80 - 140% of water holding capacity) and from pure cultures of nitrate reducing bacteria incubated with nitrate or nitrite. Gene expression analyses show a correlation of HONO emissions with imbalances in nitrite production and consumption that may result in nitrite accumulation at the cell surface. High throughput Illumina sequencing of 16S rRNA as well as nitrate and nitrite reduction associated functional gene transcripts indicate that HONO emissions from an agricultural soil sample are associated with the activity of diverse Proteobacteria. This biological process is an additional source of atmospheric HONO that should be considered in biogeochemical nitrogen cycling, global models of soil reactive nitrogen emissions, and air chemistry.
Sample scope Environment
Release date 2019-02-09
PubMed ID Article title Journal name DOI Year
30833686 Soil HONO emissions at high moisture content are driven by microbial nitrate reduction to nitrite: tackling the HONO puzzle The ISME Journal 10.1038/s41396-019-0379-y 2019
Submitter Dianming    Wu  (
Organization East China Normal University
Submission date 2017-08-27

Project Data

Resource name Description
BioSample (1) -
SAMC016304 HONO denitrification
GSA (1) -
CRA000459 HONO denitrification