Identifier PRJCA000378
Title Droplet-Cirseq
Project data type Whole genome sequencing
Organism Escherichia coli
Enterobacteria phage phiX174 sensu lato
Description We developed a method namely Droplet-CirSeq to significantly improve the amplification bias, which presents obvious superiority over the currently prevalent methods in exploitation of ultra-low frequency mutations. Droplet-CirSeq would be promisingly used in the identification of low frequency mutations initiated from extremely low input DNA, such as DNA of uncultured microorganisms, captured DNA of target region, circulation DNA of plasma et al, and its creative conception of rolling circle amplification in droplets would also be used in other low input DNA amplification fields.
Sample scope Monoisolate
Release date 2017-03-20
Publication
PubMed ID Title Journal Doi Year
PMC4784281
Submitter Kaile  Wang  (llee90504452@gmail.com)
Affiliation Beijing Institute of Genomics, Chinese Academy of Sciences
Submission date 2017-03-20

Project Data

Resource name Description
BioSample (5) -
SAMC012206 Phix174
SAMC012205 E.coli-DH5a
SAMC012204 E.coli-W3110
SAMC012203 E.coli mixture (W3110 and DH5a)-1_100
SAMC012202 E.coli mixture (W3110 and DH5a)
GSA (1) -
CRA000212 CRA000212