Accession PRJCA000262
Title Identification of full-length cDNA in Rice by PacBio sequencing
Relevance Agricultural
Data types Transcriptome or Gene expression
Organisms Oryza sativa
Description To exploit the advantage of PacBio sequencing’s long reads as possible in full-length transcripts study, we used Hiseq2000 paired-end reads to correct CCSs with different minimum predicated accuracy (MPA) and validated the full-length splicing isoforms by verifying the chained splicing junctions with at least 2 uniquely mapped Hiseq2000 reads.
Sample scope Monoisolate
Release date 2018-12-10
Publication
PubMed ID Article title Journal name DOI Year
30288819 PacBio full-length cDNA sequencing integrated with RNA-seq reads drastically improves the discovery of splicing transcripts in rice The Plant Journal 10.1111/tpj.14120 2019
Submitter Zhang    bing  (zhangbing@big.ac.cn)
Organization Chinese Academy of Sciences
Submission date 2016-08-16

Project Data

Resource name Description
BioSample (1) -
SAMC006991 Oryza sativa seedling
GSA (1) -
CRA000124 CRA_124