Identifier PRJCA000241
Title 3D Chromatin Structures of Mature Gametes and Structure Reprogramming duringMammalian Embryogenesis
Project data type Epigenomics
Organism Mus musculus
Mus musculus cytomegalovirus 2
Homo sapiens
Description The dynamics of 3D chromatin structures during mammalian embryo development remains limited known. We report the 3D chromatin architectures of mouse gametes and early embryos using an optimized Hi-C method with low-cell samples. We find that the mature oocytes at metaphase II stage do not have topologically associated domains. In sperm, the extra-long range interactions (>4 mega bases) and interchromosomal interactions are more frequent. The high-order structures of both the paternal and maternal genomes in zygotes and 2-cell embryos are obscure. The chromatin structures are gradually re-established through development. We find that the unmethylatedCpGs are enriched in A compartment, and the decreased levels of CpG methylation in A compartment are higher than those in B compartment in embryos. Furthermore, the establishment of TAD structure requires DNA replication in 2-cell embryos, but not zygotic genome activation. In summary, the global reprogramming of the chromatin architecture occurs during early mammalian development.
Sample scope Multiisolate
Release date 2017-07-13
PubMed ID Title Journal Doi Year
28709003 3D Chromatin Structures of Mature Gametes and Structural Reprogramming during Mammalian Embryogenesis. Cell 10.1016/j.cell.2017.06.029 2017
Submitter Xuepeng  Chen  (
Affiliation BIG,CAS
Submission date 2016-06-01

Project Data

Resource name Description
BioSample (34) -
SAMC013116 4-Cell Replicate2
SAMC013115 2-cell (aphidicolin treated) Hi-C R2
SAMC013114 4-Cell Replicate3
SAMC013113 HoxB b viewpoint E7.5 4Cseq
SAMC013112 HoxB a viewpoint E7.5 4Cseq
SAMC013111 HoxB b viewpoint sperm 4Cseq
SAMC013110 HoxB a viewpoint sperm 4Cseq
SAMC013109 2-Cell (alpha-amanitin treatment) Replicate2
SAMC013108 Zygote Replicate2
SAMC013103 human sperm Hi-C
SAMC013102 2-Cell (aphidicolin treated) Replicate1
SAMC007622 2-Cell (alpha-amanitin treated) RNA-seq
SAMC007621 2-Cell Control RNA-seq
SAMC007586 E7.5 Replicate4
SAMC007585 E7.5 Replicate3
SAMC007584 Zygote Replicate1
SAMC007582 2-Cell (alpha-amanitin treated) Replicate1
SAMC007581 8-Cell Replicate3
SAMC007580 4-Cell Replicate1
SAMC006432 E7.5 RNA-seq
SAMC006431 E3.5 RNA-seq
SAMC006296 E3.5 Replicate3
SAMC006295 E7.5 Replicate2
SAMC006294 E7.5 Replicate1
SAMC006293 E3.5 Replicate2
SAMC006292 E3.5 Replicate1
SAMC006291 8-Cell Replicate2
SAMC006290 8-Cell Replicate1
SAMC006289 2-Cell Replicate1
SAMC006288 2-Cell Replicate2
SAMC006287 Sperm Replicate2
SAMC006286 Sperm Replicate1
SAMC006285 MII oocyte Replicate2
SAMC006284 MII oocyte Replicate1
GSA (1) -
CRA000108 CRA000108