| Accession | PRJCA000241 | |||||||||||||||
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| Title | 3D Chromatin Structures of Mature Gametes and Structure Reprogramming duringMammalian Embryogenesis | |||||||||||||||
| Relevance | Model organism | |||||||||||||||
| Data types | Epigenomics | |||||||||||||||
| Organisms |
Mus musculus
Mus musculus cytomegalovirus 2 Homo sapiens |
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| Description | The dynamics of 3D chromatin structures during mammalian embryo development remains limited known. We report the 3D chromatin architectures of mouse gametes and early embryos using an optimized Hi-C method with low-cell samples. We find that the mature oocytes at metaphase II stage do not have topologically associated domains. In sperm, the extra-long range interactions (>4 mega bases) and interchromosomal interactions are more frequent. The high-order structures of both the paternal and maternal genomes in zygotes and 2-cell embryos are obscure. The chromatin structures are gradually re-established through development. We find that the unmethylatedCpGs are enriched in A compartment, and the decreased levels of CpG methylation in A compartment are higher than those in B compartment in embryos. Furthermore, the establishment of TAD structure requires DNA replication in 2-cell embryos, but not zygotic genome activation. In summary, the global reprogramming of the chromatin architecture occurs during early mammalian development. | |||||||||||||||
| Sample scope | Multiisolate | |||||||||||||||
| Release date | 2017-07-13 | |||||||||||||||
| Publication |
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| Submitter | Xuepeng Chen (chenxp@big.ac.cn) | |||||||||||||||
| Organization | BIG,CAS | |||||||||||||||
| Submission date | 2016-06-01 |