Accession PRJCA000241
Title 3D Chromatin Structures of Mature Gametes and Structure Reprogramming duringMammalian Embryogenesis
Relevance Model organism
Data types Epigenomics
Organisms Mus musculus
Mus musculus cytomegalovirus 2
Homo sapiens
Description The dynamics of 3D chromatin structures during mammalian embryo development remains limited known. We report the 3D chromatin architectures of mouse gametes and early embryos using an optimized Hi-C method with low-cell samples. We find that the mature oocytes at metaphase II stage do not have topologically associated domains. In sperm, the extra-long range interactions (>4 mega bases) and interchromosomal interactions are more frequent. The high-order structures of both the paternal and maternal genomes in zygotes and 2-cell embryos are obscure. The chromatin structures are gradually re-established through development. We find that the unmethylatedCpGs are enriched in A compartment, and the decreased levels of CpG methylation in A compartment are higher than those in B compartment in embryos. Furthermore, the establishment of TAD structure requires DNA replication in 2-cell embryos, but not zygotic genome activation. In summary, the global reprogramming of the chromatin architecture occurs during early mammalian development.
Sample scope Multiisolate
Release date 2017-07-13
Publication
PubMed ID Article title Journal name DOI Year
Key role for CTCF in establishing chromatin structure in human embryos Nature 10.1038/s41586-019-1812-0 2019
28709003 3D Chromatin Structures of Mature Gametes and Structural Reprogramming during Mammalian Embryogenesis. Cell 10.1016/j.cell.2017.06.029 2017
Submitter Xuepeng    Chen  (chenxp@big.ac.cn)
Organization BIG,CAS
Submission date 2016-06-01

Project Data

Resource name Description
BioSample (34)  show -
GSA (1) -
CRA000108 CRA_108