Introduction

We report a method for the identification and quantification of glycerophospholipid molecular species that is based on the simultaneous automated acquisition and processing of 41 precursor ion spectra, specific for acyl anions of common fatty acids moieties and several lipid class-specific fragment ions. Absolute quantification of identified species was linear within a concentration range of 10 nM-100 microM and was achieved by spiking into total lipid extracts a set of synthetic lipid standards with diheptadecanoyl (17:0/17:0) fatty acid moieties, representing six common classes of glycerophospholipids. The automated analysis of total lipid extracts was powered by a robotic nanoflow ion source and produced currently the most detailed description of the glycerophospholipidome.

Publications

  1. Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning.
    Cite this
    Ejsing CS, Duchoslav E, Sampaio J, Simons K, Bonner R, Thiele C, Ekroos K, Shevchenko A, 2006-09-01 - Analytical chemistry

Credits

  1. Christer S Ejsing
    Developer

  2. Eva Duchoslav
    Developer

  3. Julio Sampaio
    Developer

  4. Kai Simons
    Developer

  5. Ron Bonner
    Developer

  6. Christoph Thiele
    Developer

  7. Kim Ekroos
    Developer

  8. Andrej Shevchenko
    Investigator

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Summary
AccessionBT000054
Tool TypeApplication
Category
PlatformsLinux/Unix
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByAndrej Shevchenko